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Phlebotomy Order Of Draw

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As you may already know, blood must be drawn and collected in tubes in a specific order, known as the Order of Draw.

This is necessary to avoid cross-contamination of additives between the tubes.

Unfortunately, this order can be hard to remember for those in training and even those in the field.

We’ve recognized this and created a handy guide using multiple proven study techniques.

*Tip: Bookmark this page for future reference by pinning it as an image!

Everybody effectively learns and remembers in different ways. So, we designed several learning tools below to help you learn the proper order of draw and easily remember it in the field.

*Tip: If you're looking for a pocket-sized guide to carry around with you in the field that gives you quick access to the Order of Draw and other vital information then you have to check out the best-selling Phlebotomy Notes: Pocket Guide To Blood Collection.

Order Of Draw Chart

Blood Draw Order Tube Color Description
1 Color Varies Blood Cultures
2 Light Blue Sodium Citrate
3 Red Clot Activator
4 Gold SST
5 Light Green Lithium Heparin
6 Dark Green Sodium Heparin
7 Lavender EDTA
8 Gray Sodium Flouride
9 Yellow ACD Solution

Sources: [1], [2]

Order Of Draw Acronym

Sometimes the trick to remembering a lot of information is to create a mnemonic acronym.

This helps break the information down into easy to remember chunks, with just enough info in each chunk to help you remember the bigger picture.

Mnemonic acronyms are not set in stone, and you can create your own acronym to help you easily remember the order of blood draw.

Below is a simple and easy to remember acronym you can put to use right away. This will help you remember the blood draw order along with the tube color when performing venipuncture.

An example of this could be "Bunnies Love Roaming Gardens, Looking for Delicious Leafy Greens. Yum!"

Bunnies – Blood Culture
Love – Light Blue
Roaming – Red
Gardens – Gold
Looking (for) – Light Green
Delicious – Dark green
Leafy – Lavender
Greens – Gray
Yum – Yellow

Feel free to switch it up to make it easy to remember for YOU.

Order Of Draw Infographic

We've compiled the above information in an easy-to-understand infographic for you to easily save or share.

Phlebotomy Order Of Draw

Order Of Draw Quiz

Use the chart below to quiz yourself on the proper order of draw.

Click the “Show/Hide All” button to hide the tube color and descriptions. Then, simply go down the list in order and recite each tube color and its respective description. To check your answer, simply click on the respective color and description cell (the grayed out box to the right of the order draw number) and it will appear. Good luck!

Blood Draw Order Tube Color/Description
1 Color Varies – Blood Cultures
2 Light Blue – Sodium Citrate
3 Red – Clot Activator
4 Gold – SST
5 Light Green – Lithium Heparin
6 Dark Green – Sodium Heparin
7 Lavender – EDTA
8 Gray – Sodium Flouride
9 Yellow – ACD Solution

Reviewed on September 22, 2023